Food Products Research Articles

The Food and Nutrition Policy Environment and Drivers of Changes in Key Food System Outcomes in Ethiopia

Background: There seems to be huge gap in our understanding of the changes over time in food system outcomes and their drivers in Ethiopia. The main aim of this study is to examine the food and nutrition programs and policies and their corresponding key food system outcomes in Ethiopia. Methods: The bulk of the information was generated using scooping review of relevant articles and policy documents. About 67 full text records were used for the review. In addition, data were collected using Key Informant Interview (KIIs) purposefully selected from sectoral offices from two major cities (Hawassa and Dire Dawa), of two regions. The analytical framework used in this paper was adopted from previous studies on related subjects and addressed three key components of food system: review of food and nutrition policy environment, key food system outcomes and key drivers. Results: Despite improvement in some food system outcomes (such as child nutrition and survival), food security crises in Ethiopia are still becoming more frequent and more acute, affecting the poor disproportionately. Most food and nutrition policies are constrained by lack of implementation capacities. Indicating the presence of various barriers (socioeconomic, demographic, and environmental). Poor human capital (such as knowledge and attitude), food taboos and tradition, cultural practices such as gender-based norms, poor education, poor delivery/supply chain, demographic pressure and other environmental drivers play critical role in food and nutrition security of most vulnerable population groups in Ethiopia. Conclusion and implications: Given the challenges confronting Ethiopia today, it is imperative to assume that meeting Sustainable Development Goal (SDG) 2 (i.e., attaining zero hunger by 2030) becomes challenging. This calls for continuous capacity building to help implement, learn, and adapt a systems approach; and access to education and skill training on food production and consumption and narrowing down the gender differential in food access and consumption.

Decoding food reactions: a detailed exploration of food allergies vs. intolerances and sensitivities.

The food matrix is a complex system encompassing all constituent elements in food production. It influences the digestibility of these elements through direct interactions and affects the digestive environment. Furthermore, the gastrointestinal system possesses precise mechanisms that efficiently process dietary components into essential nutrients, effectively preventing the onset of abnormal immune responses or dysfunctional host reactions in most instances. However, the incidence of adverse food reactions is constantly increasing, and evidence indicates that this process is environmental. Adverse reactions can be categorized as toxic or nontoxic. Toxic reactions are dose-dependent and can result from natural compounds, processing-induced substances, or contaminants. Nontoxic reactions like food intolerance and hypersensitivity depend on individual susceptibility and evoke specific pathological and physiological responses. This review aims to elucidate the mechanisms underlying the occurrence of immune- (food allergies and sensitivities) and non-immune-mediated (food intolerance) reactions, emphasizing the fundamental distinctions between these two categories. Enhanced comprehension and distinction of these mechanisms will significantly contribute to advancing preventive and therapeutic approaches and establishing guidelines for food labeling concerning immune-mediated reactions.

The Food and Nutrition Policy Environment and Drivers of Changes in Key Food System Outcomes in Ethiopia

Background: There seems to be huge gap in our understanding of the changes over time in food system outcomes and their drivers in Ethiopia. The main aim of this study is to examine the food and nutrition programs and policies and their corresponding key food system outcomes in Ethiopia. Methods: The bulk of the information was generated using scooping review of relevant articles and policy documents. About 67 full text records were used for the review. In addition, data were collected using Key Informant Interview (KIIs) purposefully selected from sectoral offices from two major cities (Hawassa and Dire Dawa), of two regions. The analytical framework used in this paper was adopted from previous studies on related subjects and addressed three key components of food system: review of food and nutrition policy environment, key food system outcomes and key drivers. Results: Despite improvement in some food system outcomes (such as child nutrition and survival), food security crises in Ethiopia are still becoming more frequent and more acute, affecting the poor disproportionately. Most food and nutrition policies are constrained by lack of implementation capacities. Indicating the presence of various barriers (socioeconomic, demographic, and environmental). Poor human capital (such as knowledge and attitude), food taboos and tradition, cultural practices such as gender-based norms, poor education, poor delivery/supply chain, demographic pressure and other environmental drivers play critical role in food and nutrition security of most vulnerable population groups in Ethiopia. Conclusion and implications: Given the challenges confronting Ethiopia today, it is imperative to assume that meeting Sustainable Development Goal (SDG) 2 (i.e., attaining zero hunger by 2030) becomes challenging. This calls for continuous capacity building to help implement, learn, and adapt a systems approach; and access to education and skill training on food production and consumption and narrowing down the gender differential in food access and consumption.

Rock Phosphate and Biochar Effects on Greenhouse Gas Emissions and Soil Fertility in Southern Alberta Potato Field

The application of biochar soil amendment and rock phosphate fertilizer to the soil could lead to achieving net zero emissions and food security; however, the effectiveness of using biochar and rock phosphate in southern Alberta brown chernozemic soil is not yet assessed. In the first trial of this study, varying levels of urea (N), with varying levels of biochar (B) were applied. The same level of rock phosphate (RP) and triple super phosphate (TSP) were applied to compare efficiency of the two fertilizers. The second trial involved applying the same nutrients, but with higher levels than the first trial. The results indicated that the fertilizer application rates did not affect the growth and yield of potatoes because of the application rates of the fertilizer and fertility status of the soil, whereas in the second trial, TSP and RP treatments had the same growth and yield. Furthermore, the high application rate of nitrogen fertilizer and RP with or without biochar in the potato plot emitted high nitrous oxide gas emissions while the low application rate of nitrogen fertilizer and RP kept the carbon dioxide in the soil. Nevertheless, in the second experiment, the application of biochar and rock phosphate reduced nitrous oxide and carbon dioxide emissions. We observed high residual nitrogen and manganese nutrients after harvest in the plot treated to rock phosphate and biochar. Therefore, rock phosphate and biochar have the potential to increase food production and mitigate climate change.

Listeria monocytogenes from Food Products and Food Associated Environments: Antimicrobial Resistance, Genetic Clustering and Biofilm Insights

Listeria monocytogenes from Food Products and Food Associated Environments: Antimicrobial Resistance, Genetic Clustering and Biofilm Insights

Uneven agricultural contraction within fast-urbanizing urban agglomeration decreases the nitrogen use efficiency of crop production.

Diverse development paths among cities within an urban agglomeration can lead to uneven changes in their agricultural production scale, which reshape the inter-city food supply patterns and the spatiotemporal characteristics of nitrogen (N) pollution from the food system. Here, using Guangdong-Hong Kong-Macao Greater Bay Area of China as a case, we found a substantial decrease in N use efficiency of crop production from 45.2% to 29.3% during 1989-2007, along with a growing level of concentration of food N production in less-urbanized cities. From 1989 to 2018, 12.3% to 42.2% of total N pollution in food production became embedded in inter-city trade, leading to aggregation of N pollution in peripheral cities with relatively low levels of economic development. We suggest that protection and intensification of cropland from urban encroachment, as well as enhancing the economic and technical synergies among cities, can serve the sustainable transition of the food system with coordinated N pollution mitigation.

New insight into protective effect against oxidative stress and biosynthesis of exopolysaccharides produced by Lacticaseibacillus paracasei NC4 from fermented eggplant.

Fermented eggplant is a traditional fermented food, however lactic acid bacteria capable of producing exopolysaccharide (EPS) have not yet been exploited. The present study focused on the production and protective effects against oxidative stress of an EPS produced by Lacticaseibacillus paracasei NC4 (NC4-EPS), in addition to deciphering its genomic features and EPS biosynthesis pathway. Among 54 isolates tested, strain NC4 showed the highest EPS yield and antioxidant activity. The maximum EPS production (2.04 ± 0.11g/L) was achieved by culturing in MRS medium containing 60g/L sucrose at 37°C for 48h. Under 2mM H2O2 stress, the survival of a yeast model Saccharomyces cerevisiae treated with 0.4mg/mL NC4-EPS was 2.4-fold better than non-treated cells, which was in agreement with the catalase and superoxide dismutase activities measured from cell lysates. The complete genome of NC4 composed of a circular chromosome of 2,888,896bp and 3 circular plasmids. The NC4 genome comprises more genes with annotated function in nitrogen metabolism, phosphorus metabolism, cell division and cell cycle, and iron acquisition and metabolism as compared to other reported L. paracasei. Of note, the eps gene cluster is not conserved across L. paracasei. Pathways of sugar metabolism for EPS biosynthesis were proposed for the first time, in which gdp pathway only present in few plant-derived bacteria was identified. These findings shed new light on the cell-protective activity and biosynthesis of EPS produced by L. paracasei, paving the way for future efforts to enhance yield and tailor-made EPS production for food and pharmaceutical industries.

Editorial: Technologies to address risk assessment, food safety and public health in food production chain

Editorial: Technologies to address risk assessment, food safety and public health in food production chain

Improving Indigenous Food Sovereignty through sustainable food production: a narrative review

Improving Indigenous Food Sovereignty through sustainable food production: a narrative review

The application of FTIR spectra coupled with chemometrics for analysis of lard in food products for halal authentication: a mini review

Lard (LD) obtained from fat’s extraction of adipose tissue of swine is a good component to be used in food and pharmaceutical products to provide specific functions. Lard, according to the Food and Drug Administration, is generally considered recognized as safe (GRAS) to be used in the products. However, LD is not allowed to be consumed or used in the products due to certain religious restrictions in which Muslims and Jews are not allowed to consume any products containing LD because LD is categorized as nonhalal (Muslims) and non-kosher (Jews). Thus, the analysis of LD in food and pharmaceutical products is very urgent to ensure the product’s halalness (and Kosherness) for Muslims and Jews. Some methods have been reported for the analysis of LD, including FTIR spectra, due to their property as fingerprinting analytical techniques capable of fast and reliable tools for screening LD. The responses obtained from the measurement of LD using FTIR spectroscopy are very large, involving big data analysis. Therefore, the chemometrics application to treat these chemical data is unavoidable. This present review highlighted the use of FTIR spectra and chromatographic-based techniques coupled with chemometrics for the analysis of lard in food and pharmaceutical products for halal authentication. The main findings of this review can assist regulators and analytical chemists in selecting the best method for analysis of LD in the products intended for Halal authentication analysis.

The hydrates of chlorogenic acid in its aqueous solution and in stored food products

AbstractChlorogenic acids (CQAs), a large family of naturally occurring esters of quinic acid with one, two or even three cinnamic acids moieties and/or moieties of cinnamic acids derivatives, supplied to human organisms mainly with coffee, tea, fruit and vegetables, have been among the most frequently studied polyphenols. Of these, caffeic acid esters predominate, and 5-O-caffeoylquinic acid (5-CQA) is their main and most widespread representative. Recently performed studies have shown that after removing water from the aqueous solution of 5-CQA, its dry residue contains two hydrates of this compound, each consisting of two 5-CQA molecules and two molecules of H2O (2 × 5-CQA*2xH2O). The results presented in the paper not only define the characteristic features of 5-CQA but, more importantly, offer solid evidence that 5-CQA hydrates begin to form already in the aqueous solution of 5-CQA immediately after the dissolution of this compound and that the analogous process can occur in food products containing 5-CQA during their storage. Moreover, the performed experiments indicate the validity of the hypothesis that 5-CQA hydrates are formed by active collisions of 5-CQA molecules with monomeric/dimeric water molecules existing in trace amount in strongly associated aqueous systems. The presence of 5-CQA hydrates in liquid food products may have a significant impact on the assessment of some of their physicochemical properties as well as their biochemical activity.

Linking farmers’ perceptions and management decision toward sustainable agroecological transition: evidence from rural Tunisia

Global food systems face sustainability challenges like undernourishment, inequity, resource degradation, and pollution. Food production and consumption drive environmental change with greenhouse gas emissions, biodiversity loss, and land-system shifts. The climate change crisis has intensified concerns about the ecological impact of these systems. Sustainable food networks, such as community-supported agriculture, are promoting sustainable production and consumption through short supply chains. International bodies like the Food and Agriculture Organization (FAO) and the Consultative Group for International Agricultural Research (CGIAR) are also spearheading initiatives for more equitable and sustainable food systems. In Tunisia, where dryland areas predominate, the ongoing implementation of the Agroecology Initiative provides the context for this study, which explores the drivers and barriers of agroecological transformation in this challenging environment. The research focuses on stakeholder engagement, with a gender perspective to explore farmer perceptions. The study, conducted in the northwest of Tunisia in 2022–2023, involved focus groups, workshops, surveys, and questionnaires with various stakeholders. Findings highlight farmer organizations’ potential in promoting sustainable farming, with clear goals, diversified systems, and collaborations. However, challenges such as input scarcity, water shortage, low income, and marketing must be addressed. Results also indicate that over 90% of farmers who received assistance with agroecological practices reported a change in their ideas and practices. Fifty seven percent of the workshops participants identified the olive oil value chain as having the greatest potential for agroecological transformation, but it faces constraints such as climate, lack of policy incentives, training, funding, and difficulty in adopting technical innovations. Women’s inclusion in agriculture, environmental, social, and economic challenges were also highlighted. Despite these obstacles, key drivers for agroecological transition were identified. These include the compatibility of many agroecological practices with existing farmer capabilities, their cultural and economic benefits, and the positive outcomes for environmental sustainability and health. The study advocates for a socio-technical systems analysis to address the root causes hindering Tunisia’s agroecological transformation. A participatory approach is crucial to understanding priorities and developing a sustainable and resilient food system. Furthermore, the research underscores the importance of considering diverse farmer perspectives and tailoring strategies to support this critical transition effectively.

Biological Activity and Phytochemical Characteristics of Star Anise (Illicium verum) Essential Oil and Its Anti-Salmonella Activity on Sous Vide Pumpkin Model

Illicium verum, commonly known as star anise, represents one of the notable botanical species and is recognized for its rich reservoir of diverse bioactive compounds. Beyond its culinary application as a spice, this plant has been extensively utilized in traditional medicine. Given the contemporary emphasis on incorporating natural resources into food production, particularly essential oils, to enhance sensory attributes and extend shelf life, our study seeks to elucidate the chemical composition and evaluate the antibacterial (in vitro, in situ) and insecticidal properties of Illicium verum essential oil (IVEO). Also, microbiological analyses of pumpkin sous vide treated with IVEO after inoculation of Salmonella enterica were evaluated after 1 and 7 days of study. GC/MS analysis revealed a significantly high amount of (E)-anethole (88.4%) in the investigated EO. The disc diffusion method shows that the antibacterial activity of the IVEO ranged from 5.33 (Streptococcus constellatus) to 10.33 mm (Citrobacter freundii). The lowest minimal inhibition concentration was found against E. coli and the minimum biofilm inhibition concertation was found against S. enterica. In the vapor phase, the best antimicrobial activity was found against E. coli in the pears model and against S. sonei in the beetroot model. The application of the sous vide method in combination with IVEO application decreased the number of microbial counts and eliminated the growth of S. enterica. The most isolated microbiota identified from the sous vide pumpkin were Bacillus amyloliquefaciens, B. cereus, B. licheniformis, and Ralstonia picketii. Modifications to the protein composition of biofilm-forming bacteria S. enterica were suggested by the MALDI TOF MS instigations. The IVEO showed insecticidal potential against Harmonia axyridis. Thanks to the properties of IVEO, our results suggest it can be used in the food industry as a natural supplement to extend the shelf life of foods and as a natural insecticide.

Raman and Mid-Infrared Spectroscopy Coupled With Machine–Deep Learning for Adulterant Detection in Ground Turmeric

The intricate nature of the global food supply chain and the presence of regulations spanning multiple jurisdictions contribute to an increased likelihood of food adulteration. This underscores the need for effective monitoring methods to guarantee the safety and nutritional quality of our food. In this context, the application of infrared spectroscopy-based techniques emerges as an environmentally friendly, non-invasive, and waste-minimizing solution for authenticating food products. Infrared spectra serve as unique molecular fingerprints, offering a multidimensional representation of how chemical bonds in the material interact with infrared light. Chemometrics, which are primarily linear-based models, play a crucial role in extracting essential information from spectral data, enabling dimensionality reduction, classification, and predictive analysis. Recent progress in the field of big data science and artificial intelligence has brought forth machine learning and deep learning algorithms explicitly designed to uncover features from complex multidimensional data, encompassing both linear and nonlinear relationships. These advancements have the potential to enhance the detection of adulterants in food products. This study assesses the accuracy of various shallow machine learning models and a deep learning model based on a one-dimensional convolutional neural network (1D CNN). The evaluation is conducted using Raman and infrared spectral data obtained from ground turmeric samples that were deliberately adulterated with five distinct substances. The study highlights the improved classification accuracy achieved through the implementation of the 1D CNN model.

Mimicking natural deterrent strategies in plants using adhesive spheres

With a continuous increase in world population and food production, chemical pesticide use is growing accordingly, yet unsustainably. As chemical pesticides are harmful to the environment and developmental resistance in pests is increasing, a sustainable and effective pesticide alternative is needed. Inspired by nature, we mimic one defense strategy of plants, glandular trichomes, to shift away from using chemical pesticides by moving toward a physical immobilization strategy via adhesive particles. Through controlled oxidation of a biobased starting material, triglyceride oils, an adhesive material is created while monitoring the reactive intermediates. After being milled into particles, nanoindentation shows these particles to be adhesive even at low contact forces. A suspension of particles is then sprayed and found to be effective at immobilizing a target pest, thrips, Frankliniella occidentalis. Small arthropod pests, like thrips, can cause crop damage through virus transfer, which is prevented by their immobilization. We show that through a scalable fabrication process, biosourced materials can be used to create an effective, sustainable physical pesticide.

Hexagonal Boron Nitride Quantum Dots Embedded on Layer-by-Layer Films for Peroxidase-Assisted Colorimetric Detection of β-Galactosidase Producing Pathogens.

Pathogen detection has become a major research area all over the world for water quality surveillance and microbial risk assessment. Therefore, designing simple and sensitive detection kits plays a key role in envisaging and evaluating the risk of disease outbreaks and providing quality healthcare settings. Herein, we have designed a facile and low-cost colorimetric sensing strategy for the selective and sensitive determination of β-galactosidase producing pathogens. The hexagonal boron nitride quantum dots (h-BN QDs) were established as a nanozyme that showed prominent peroxidase-like activity, which catalyzes 3,3',5,5'-tetramethylbenzidine (TMB) oxidation by H2O2. The h-BN QDs were embedded on a layer-by-layer assembled agarose biopolymer. The β-galactosidase enzyme partially degrades β-1,4 glycosidic bonds of agarose polymer, resulting in accessibility of h-BN QDs on the solid surface. This assay can be conveniently conducted and analyzed by monitoring the blue color formation due to TMB oxidation within 30 min. The nanocomposite was stable for more than 90 days and was showing TMB oxidation after incubating it with Escherichia coli (E. coli). The limit of detection was calculated to be 1.8 × 106 and 1.5 × 106 CFU/mL for E. coli and Klebsiella pneumonia (K. pneumonia), respectively. Furthermore, this novel sensing approach is an attractive platform that was successfully applied to detect E. coli in spiked water samples and other food products with good accuracy, indicating its practical applicability for the detection of pathogens in real samples.

Solid-state fermentation using wheat bran to produce glucose syrup and functional cereal bars.

Wheat bran is one of the most abundant by-products from grain milling, which can be used as substrate for solid-state fermentation (SSF) to obtain enzymes able to convert this agro-industrial waste into glucose syrup, which in turn can be applied for the production of different food products. The present study aimed to determine centesimal composition of wheat bran, obtain enzymatic extract that converts wheat bran into wheat glucose syrup (WGS), produce rice flakes cereal bars (RFCB), and evaluate their nutritional composition and the presence of functional compounds, as well as their antioxidant potential. Determination of centesimal composition of wheat bran demonstrated its nutritional potential. Enzymatic extract was obtained and it converted wheat bran into WGS, which were applied to rice flakes producing RFCB. These cereal bars proved to be a source of dietary fiber (1.8g) and soluble protein (7.2g) while RCFB produced with corn glucose syrup did not present these nutritional components. In addition, RFCB produced with WGS showed polyphenolic compounds, among them flavonoids, which exhibited antioxidant activity by DPPH and ABTS radical scavenging (47.46% and 711.89μM Trolox Equivalent/g, respectively), and iron ion reduction (71.70μM Trolox equivalent/g). Final product showed a decrease in caloric value and sodium content. Therefore, the present study showed that the bioprocess of SSF yields a nutritional, ecological, and functional food product, which might be of great interest for food industry, adding nutritional and functional value to a well-stablished product.

Fusarium mycotoxins: The major food contaminants

AbstractMycotoxins, which are secondary metabolites produced by toxicogenic fungi, are natural food toxins that cause acute and chronic adverse reactions in humans and animals. The genus Fusarium is one of three major genera of mycotoxin‐producing fungi. Trichothecenes, fumonisins, and zearalenone are the major Fusarium mycotoxins that occur worldwide. Fusarium mycotoxins have the potential to infiltrate the human food chain via contamination during crop production and food processing, eventually threatening human health. The occurrence and development of Fusarium mycotoxin contamination will change with climate change, especially with variations in temperature, precipitation, and carbon dioxide concentration. To address these challenges, researchers have built a series of effective models to forecast the occurrence of Fusarium mycotoxins and provide guidance for crop production. Fusarium mycotoxins frequently exist in food products at extremely low levels, thus necessitating the development of highly sensitive and reliable detection techniques. Numerous successful detection methods have been developed to meet the requirements of various situations, and an increasing number of methods are moving toward high‐throughput features. Although Fusarium mycotoxins cannot be completely eliminated, numerous agronomic, chemical, physical, and biological methods can lower Fusarium mycotoxin contamination to safe levels during the preharvest and postharvest stages. These theoretical innovations and technological advances have the potential to facilitate the development of comprehensive strategies for effectively managing Fusarium mycotoxin contamination in the future.

Extent of alignment between the Australian Dietary Guidelines and the NOVA classification system across the Australian packaged food supply.

The Australian Dietary Guidelines are currently being revised and ultra-processed foods have been identified as a high priority action area. To better understand how well the current Dietary Guidelines align with level of processing classifications, the aim of this study was to assess the alignment between the Australian Dietary Guidelines and the NOVA classification system for classifying the healthiness of packaged foods. Data were sourced from the Australian FoodSwitch dataset, which included 28 071 packaged food and beverage products available in major Australian supermarkets in 2022. Products were classified as (i) core or discretionary (Australian Dietary Guidelines) and (ii) non-ultra-processed or ultra-processed (NOVA). Agreement between the two systems (core vs. non-ultra-processed and discretionary vs. ultra-processed) was evaluated using the kappa statistic. There was 'moderate' agreement (κ = 0.41, 95% CI: 0.40-0.42) between the Australian Dietary Guidelines and the NOVA system, with 69.8% of products aligned across the two systems. Alignment was more common for discretionary foods (80.6% were ultra-processed) than core foods (59.9% aligned were not-ultra-processed). Food categories exhibiting the strongest levels of alignment included confectionary, foods for specific dietary use, and egg and egg products. Discordance was most common for convenience foods, sugars, honey and related products, and cereal and grain products. Despite moderate alignment between the Australian Dietary Guidelines and NOVA, the discordance observed for almost one-third of products highlights the opportunity to develop recommendations for ultra-processed foods within the guidelines to advise Australians how these foods should be considered as part of a healthy diet.

The Contribution of Subsistence Agriculture to the Livelihoods of the Smallholder Farmers in South Kivu Province, Democratic Republic of Congo

Subsistence agriculture in most cases is practiced on a limited space of land by farmers and it is dependent on human power that results into production of food consumed at household level, with little or no surplus for sale. A study about the contribution of subsistence agriculture to the livelihoods of the smallholder farmers in south Kivu province was carried out. A total of 303 smallholder farmers were randomly selected and interviewed to examine the effects of practicing subsistence agriculture on the socioeconomic status of the farmers. How the socioeconomic status attained by the farmers influenced their sustainable livelihoods was also assessed. Data were analyzed using means, frequency distribution, correlation, ANOVA and Chi square test. Thematic analysis was done on qualitative data. The farmers practiced shifting and primitive agriculture, intensive subsistence, and nomadic herding. There was a significant difference in the yields of crops grown (F = 0.0088; P = 0.05), while education and income status significantly affected the farmer’s socioeconomic status by practicing subsistence agriculture (P < 0.05). An average mean value of 3.12 for the components of the farmers’ livelihoods were exhibited, with the human capital mean value being highest (4.16) compared to natural, social and financial capital. The socioeconomic status attained had a significant effect on sustainable livelihoods of the farmers at P < 0.05. This implies that the smallholder farmers need to be trained by the agricultural extension workers better and sustainable methods of farming for higher crop yields. In addition, the Central Government though the Ministry of Agriculture, should avail improved seed inputs to the local community for better crop yields. All these will enable the smallholder farmers improve their socioeconomic status and at the same time attain higher sustainable livelihoods.